Such a memorandum was unprecedented for the Bolshevik regime and the response by them was immediate. In February 1923, Patr. Ambrose and his council were arrested and imprisoned. In a public show trial, Patr. Ambrose and his fellow clergy were accused of hiding historic treasures of the Church in order to keep them from passing into the hands of the Soviet state. In his defense at the end of the trial, Patr. Ambrose stated, ''My soul belongs to God, my heart to my country; you, my executioners, do what you will with my body."
While expecting execution, the Bolsheviks did not dare to execute him and sentenced Patr. Ambrose to eight years imprisonment. His property was also confiscated. During the time of his imprisonment from 1923, Metr. Kalistrate was locum
tennes. The public outcry over the extent of the Red Terror in Georgia caused the Bolsheviks to moderate their pressure on Georgian society. In March 1926, the Bolsheviks put forward an amnesty for the 1924 insurrection and suspension of religious persecutions. Later in 1926, Ambrose and a few Georgian clergy were released from prison. However, the strains of the years showed, and Patr. Ambrose soon reposed in Tbilisi on [[March 29]], 1927.