Agni Parthene (Gr. Αγνή Παρθένε) is a non-liturgical hymn composed in Greek by St. Nectarios of Aegina in the 19th century during his tenure as director of the Rizarios Theological School of Athens. St. Nektarios liked to compose hymns in honor of the Mother of God to deepen his personal prayer and relationship with her. The tradition passed down at the monastery of St. Nektarios, in Aegina, Greece, is that the Lady herself appeared before him and requested that he record on paper a particular hymn the angelic choirs were about to sing. This hymn was the "Agni Parthene". The original script can still be viewed on his prayer table in his bedroom at this monastery.
The original melody to this hymn was composed by an Athonite hymnographer who is said to also have had a vision of the Virgin Mary prior to composing the work. The recording of this melody by the Monks of Simonopetra has contributed significantly to the popularization of the hymn.
Due to its popularity, the hymn has been translated into many langauges and the original melody has been adapted to suit the particular style of church chanting. One such variation is the Valaam Monastery chant of the Russian translation.
The hymn of the Pure Virgin is part of A Small Theotokarion (Athens, 1905) a book of Hymns to the Mother of God, written by St. Nektarios.
This hymn has been used during communion in the Liturgy and it is sometimes chanted at the beginning of Vespers.