The cathedral, now referred to as the Old Cathedral, was completed in 1593. At first the monastery numbered only a few [[monk]]s and was an appendage of the Andronikov Monastery. The monastery was overrun by Polish forces during the Time of Troubles and abandoned temporarily in the early seventeenth century . By mid-seventeenth century restoration of the monastery was underway. It was later in the century that it was enlarged and strengthen by the Regent Sophia when the monastery was needed to fend off marauding Tatars. In 1683, the [[abbot]] of the monastery was elevated to the rank of [[archimandrite]]. Construction of the New (Great) Cathedral, in a style reminiscent of that used in the Ukraine, was begun 1684 by masons and artisans from Ukraine. A sixteenth century, a copy of the Lady of the Don icon became the center piece of the eight tiered carved [[iconostasis]]. Between 1782 and 1785, the frescos in the cathedral were painted by the Italian, Antonio Claudio, the first frescos in Moscow painted by a foreigner.

The year 1771 bought riots to Moscow as the plague ravaged the city and its environs. Ambrosius, the [[Metropolitan]] of Moscow, was caught up in the riots and fled to Donskoy Monastery where the enraged crowd cornered him and bludgeoned him to death. Another fallout of the plague and the death of Metr. Ambrosius was Catherine II’s edict banning cemeteries within the limits of the city. This led to the establishment of seven cemeteries around the city, with one at Donskoy Monastery. The Donskoy cemetery became one of the most prestigious in Moscow as the great families of the city came to use the cemetery. Among these families the princely Golitsyn and Zubov families built on the monastery grounds their private chapels, the Alexander Svirsky Church and the Archangel Church respectively.